Specification:96 T/480 T/960 T Samples:Serum, plasma, tissue homogenates
Description About Canine Cortisol ELISA Kit:
Canine Cortisol ELISA Kit is based on a widely used immunoassay technique. A sample (serum/ plasma/urine) containing an unknown amount of Cortisol to be assayed (unlabeled antigen) is added to a standard amount of a labeled derivati.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for cortisol and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated cortisol and cortisol in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of cortisol in the sample. The color development is stopped and the intensity of the color is measured. Research Use Only:For Research Use Only. Not for use in diagnostic procedures.