Carbamate ELISA Kit

Use purpose:
This kit for Feed, fish, shrimp and meat tissue (such as chicken, beef and pork), eggs, honey, milk, serum and urine in the Carbamate remaining quantitative detection.
Experimental principle:
This kit adopts methods in competition ELISA In microplates coated with antigen conjugated Carbamate, add Carbamate standard or samples, Free Carbamate and pre-coated on strips of Carbamate conjugated antigen compete against Carbamate antibody conjugates, With TMB chromogenic substrate,the color is changed from blue to yellow after adding stop solution, enzyme standard instrument 450nm wavelengths in testing, absorb light value and in the sample was Carbamate content is inversely proportional to the standard curve, through calculation samples was Carbamate concentrations.
Materials provided with the kit:
1 Microelisa stripplate: 1 block (12well×8strips).
2 Carbamate standard: six vials of (1ml/ vial), content is respectively: 0 PPB, 0.1 PPB, 0.3 PPB and 0.9 PPB, 2.7 PPB, 8.1 PPB.
3 Anti- Carbamate antibody conjugate: 1 vial (6ml).
4 Chromogen Solution A: 1 vial (6ml).
5 Chromogen Solution B: 1 vial (6ml).
6 Stop Solution: 1 vial (6ml), 2M sulphuric acid.
7 sample dilution: 1 vial (10 x, 6ml), used for sample diluted with.
8 wash solution: 1 vial (20 x, 20ml), used for washing board.
9 Instruction.
Precautions:
1 please read the instructions carefully , before useing the kit.
2 don't use expired kit.
3 before using the kit, please let the reagent recover to room temperature (25 + 2 ℃), the proposal for at least 2 hours to temperature.
4 the standard contain Carbamate, pay special attention to, the operation, we should bring gloves.
5 stop solution is containing sulfate, when using, prevent burns skin and corrosion clothing.
6 different standard and sample suction head used cannot be mixed use, otherwise, it will affect test results.
7 different batches of reagent kit not mix, Different standard and sample suction head shall not be used in combination, otherwise, it will affect the experimental result.
8 diluted sample must use this kit of sample diluent, otherwise, it will affect the experimental result
9 mixed reagents should avoid blistering.