Description About Tachypleus Amebocyte Lysate(TAL):
The Tachypiens Amebocyte Lysate is a sterile freeze-drying product made of blood amoebocyte lysate of marine organisms, containing proclotting enzyme and coagulogen which are activated by microbial endotoxin and fungal glucan. The Tachypiens Amebocyte Lysate is a biological reagent obtained by extracting amoebocyte lysate from the blue blood of the limulus which is inhabited by the sea, and freeze-drying at a low temperature. It accurately and quickly determines whether a sample contains bacterial endotoxin and (1,3)-β-glucan in a qualitative or quantitative manner.
The main component of Tachypiens Amebocyte Lysate is the horseshoe crab hemocytes, which is treated with chloroform to remove the anti-lipopolysaccharide factor, and the appropriate amount of divalent calcium and magnesium ions are added, and the C, B, and G factors are contained.
At present, Tachypiens Amebocyte Lysate is widely used in the fields of pharmaceutical, clinical and scientific research for the detection of bacterial endotoxin and fungal glucan. The Amebocyte Lysates currently used are classified into Limulus Amebocyte Lysate and Tachypleus Amebocyte Lysate.
Tachypleus amebocyte lysate(TAL)or limulus amebocyte lysate(LAL)has been extensively used for bacterial endotoxin test.